منابع مشابه
Nonimmune Fluorescent Protein Staining of Neoplasms
In 1952 Weiler demonstrated with ultraviolet microscopy that anti-rat liver microsomal and mitochondrial rabbit globulin conjugated with fluorescein isocyanate became selectively attached to normal and hyperplastic rat liver but not to paradimethylamino-azobenzol-induced hepato-carcinoma within such livers. In 1956 he demonstrated similar antigenic differences between normal hamster kidney and ...
متن کاملStaining and peeling of the internal limiting membrane using a fluorescent dye (Rhodamine 6 G).
AIM To assess whether low concentrations of a fluorescent dye such as Rhodamine 6G would help the unaided human eye visualise the vitreous and the internal limiting membrane (ILM) under standard halogen illumination. MATERIAL/METHODS The UV/Vis absorption (E) and fluorescence (I) spectra of Rhodamine 6G in water were measured and compared with Indocyanine Green (ICG). Surgery was performed in...
متن کاملFluorescent DNA nanotags: supramolecular fluorescent labels based on intercalating dye arrays assembled on nanostructured DNA templates.
Fluorescence detection and imaging are vital technologies in the life sciences and clinical diagnostics. The key to obtaining high-resolution images and sensitive detection is to use fluorescent molecules or particles that absorb and emit visible light with high efficiency. We have synthesized supramolecular complexes consisting of a branched DNA template and fluorogenic intercalating dyes. Bec...
متن کاملFluorescent dye assay for detection of DNA in recombinant protein products.
A homogeneous fluorescence-based DNA detection system has been developed to measure DNA in protein solutions. The technique relies on the increase in fluorescence of a dye molecule when it intercalates into double-stranded (ds) DNA. The increased fluorescence is a direct measurement of the amount of DNA in the sample. The analysis time required per sample is less than 5 min. The dye has absorba...
متن کاملCombination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery.
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.590